| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165915 |
| Chromosome: | chromosome 10 |
| Location: | 1714292 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430450 | (1 of 1) 3.5.1.10 - Formyltetrahydrofolate deformylase / Formyltetrahydrofolate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCGCGTTGCTTGGCTAGGCCCTCATG |
| Internal bar code: | GGCATGGGCTCCGCAGTTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 99.6769 |
| LEAP-Seq n confirming: | 17274 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATGTCCACTGATACACG |
| Suggested primer 2: | ACTGCCTGGTCGATGATAGG |