| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165940 |
| Chromosome: | chromosome 13 |
| Location: | 2708893 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582250 | (1 of 1) 3.2.1.96 - Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase / Endo-beta-N-acetylglucosaminidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGGTATTCAGGCGGGCTTGGCGGCTT |
| Internal bar code: | GCGGACGTTAAGGGGAGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 66.721 |
| LEAP-Seq n confirming: | 24572 |
| LEAP-Seq n nonconfirming: | 12256 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTATATGCATGGGGTGG |
| Suggested primer 2: | GGGTTACGACACGCATACAA |