| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.165944 |
| Chromosome: | chromosome 6 |
| Location: | 3210722 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276050 | AKR2 | Aldo/keto reductase; (1 of 1) PTHR11732//PTHR11732:SF175 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTGTCCACGCGGCGCGAGGGTGCGCAG |
| Internal bar code: | TGCGGCTCCGGAACCTGACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 91.1068 |
| LEAP-Seq n confirming: | 1424 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACATGGCACGTTATGCAC |
| Suggested primer 2: | GTCTAGGAAGCGATTCAGCG |