| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165944 |
| Chromosome: | chromosome 6 |
| Location: | 3210726 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276050 | AKR2 | Aldo/keto reductase; (1 of 1) PTHR11732//PTHR11732:SF175 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGACGTAGCTTCCGAGCCTGCCGTAC |
| Internal bar code: | GTGCTAAAGGGCTACGTACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1137 |
| LEAP-Seq percent confirming: | 99.5556 |
| LEAP-Seq n confirming: | 4705 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACATGGCACGTTATGCAC |
| Suggested primer 2: | GTCTAGGAAGCGATTCAGCG |