| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165980 |
| Chromosome: | chromosome 7 |
| Location: | 3792724 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338400 | (1 of 1) K14845 - RAT1-interacting protein (RAI1, DOM3Z) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGACGTGGACGGCTTGGACTCTGGCGGC |
| Internal bar code: | GCCTTTGTTGAGGGTTTTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 99.4577 |
| LEAP-Seq n confirming: | 7153 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGGTAGAGTCAACGGC |
| Suggested primer 2: | TGTCTCTTCCTCCAGCCTGT |