| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.166001 |
| Chromosome: | chromosome 6 |
| Location: | 5400697 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283900 | (1 of 1) PTHR16007//PTHR16007:SF15 - EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED // TRANSMEMBRANE PROTEIN 45B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCTCACTTTTTACGAACTTCTCGCCAA |
| Internal bar code: | GCTACGTCCAGATAGCATGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 98.8113 |
| LEAP-Seq n confirming: | 665 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGTACATCAGCACGGA |
| Suggested primer 2: | ATGCACATACACGTCCTCCA |