| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166006 |
| Chromosome: | chromosome 1 |
| Location: | 3326472 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020918 | PREP1 | (1 of 1) PTHR11851:SF68 - PRESEQUENCE PROTEASE 1, CHLOROPLASTIC/MITOCHONDRIAL-RELATED; Presequence protease 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGTCTGGGCAGGCTGCTCCAGCCATTG |
| Internal bar code: | AGGACCTCATTCCGCAGTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 99.2927 |
| LEAP-Seq n confirming: | 1123 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCGGTCACATACACATAC |
| Suggested primer 2: | TGCCCTGCCTCTAGCTGTAT |