| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166010 |
| Chromosome: | chromosome 14 |
| Location: | 4037853 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633550 | SCP2 | (1 of 1) 2.3.1.176 - Propanoyl-CoA C-acyltransferase / Sterol carrier protein-X; Sterol carrier/transfer protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTACAGCGCCACCCGTCCTCGTGCCGCT |
| Internal bar code: | GGTAGGTTGTAGGTGGCACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 83.37 |
| LEAP-Seq n confirming: | 757 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCTTGTGCATGTACGG |
| Suggested primer 2: | GGCTGATGTGCAGCTTATGA |