Insertion junction: LMJ.RY0402.166012_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.166012
Chromosome:chromosome 2
Location:4083992
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g097550 (1 of 1) PTHR12532:SF0 - TRANSCRIPTIONAL REGULATORY PROTEIN HAH1-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATCACTCGCATGCCGCGCTCACGCTATC
Internal bar code:ACTGTTTCAACGATGAGGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:444
LEAP-Seq percent confirming:98.8994
LEAP-Seq n confirming:16534
LEAP-Seq n nonconfirming:184
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTATTGGGTGTGTCGCATGT
Suggested primer 2:GTTATTGCGTGGTTGCAATG