| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166016 |
| Chromosome: | chromosome 16 |
| Location: | 3170427 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666100 | (1 of 1) K14168 - cytoplasmic tRNA 2-thiolation protein 1 (CTU1, NCS6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGTGGTCGTCTGTTCTAGGCGCAAACTC |
| Internal bar code: | GAAGGGCCACGCCGGTACAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 99.7706 |
| LEAP-Seq n confirming: | 435 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTGCTAACACTACCAT |
| Suggested primer 2: | GTAATCAGGGCGGATCTTCA |