Insertion junction: LMJ.RY0402.166017_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.166017
Chromosome:chromosome 12
Location:2149180
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g508750 LHCA2 Light-harvesting protein of photosystem I; (1 of 24) IPR001344//IPR022796//IPR023329 - Chlorophyll A-B binding protein, plant // Chlorophyll A-B binding protein // Chlorophyll a/b binding protein domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATTACACGAGCTTTCCCCGTGCAATTTT
Internal bar code:GGAATGGTCATTTGGATTATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:689
LEAP-Seq percent confirming:99.8242
LEAP-Seq n confirming:1136
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCTTGGCAGATCAGTTA
Suggested primer 2:TGTGATCAGCTTCCAGCAAC