| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166017 |
| Chromosome: | chromosome 12 |
| Location: | 2149194 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508750 | LHCA2 | Light-harvesting protein of photosystem I; (1 of 24) IPR001344//IPR022796//IPR023329 - Chlorophyll A-B binding protein, plant // Chlorophyll A-B binding protein // Chlorophyll a/b binding protein domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTGCGAGTGCTCACACCGTACGGTAGA |
| Internal bar code: | GCGGCTAGGCTGCGACGGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1138 |
| LEAP-Seq percent confirming: | 97.3347 |
| LEAP-Seq n confirming: | 5770 |
| LEAP-Seq n nonconfirming: | 158 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCACAGTCTACACCTCGT |
| Suggested primer 2: | TGTGATCAGCTTCCAGCAAC |