| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166021 |
| Chromosome: | chromosome_8 |
| Location: | 3800537 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g377350 | FAP121,FAP180,ADK5 | Adenylate kinase; Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCCGGCGGGGCTGGGGCGTCCCCGGCCGC |
| Internal bar code: | GTGTGGTCAACAGTGGATTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 88.5922 |
| LEAP-Seq n confirming: | 365 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTAGAGCACAGTTGGCA |
| Suggested primer 2: | GTGTGAGCTGCGAAAGACAG |