Insertion junction: LMJ.RY0402.166036_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.166036
Chromosome:chromosome 6
Location:1163267
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g257250 BBS2 Bardet-Biedl syndrome-2 associated protein; (1 of 1) K16747 - Bardet-Biedl syndrome 2 protein (BBS2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCTTCGGCAGCACTACGCCCCACCAACG
Internal bar code:AGACACGAAGTCAAGTCGCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:293
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:187
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GATAGAGCTGCCCAAGTTCG
Suggested primer 2:TGGGGGCAGTGAAGAATAAG