Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.166036 |
Chromosome: | chromosome_6 |
Location: | 1163274 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g257250 | BBS2 | Bardet-Biedl syndrome-2 associated protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCCGAATGCGCACGGTGTCGAAGAGGGTG |
Internal bar code: | GCAGTGAGGTGAACTGAGCATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 509 |
LEAP-Seq percent confirming: | 99.763 |
LEAP-Seq n confirming: | 9682 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GATAGAGCTGCCCAAGTTCG |
Suggested primer 2: | TGGGGGCAGTGAAGAATAAG |