Insertion junction: LMJ.RY0402.166044_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.166044
Chromosome:chromosome 6
Location:3512776
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278107 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGTTGCGCTCTTGTTGCCGTGTGTTGCA
Internal bar code:TTGGACGCACTGAATTAAACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:92
LEAP-Seq percent confirming:99.0232
LEAP-Seq n confirming:811
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCTGTCGCTTCAGGAAC
Suggested primer 2:CCTCAAGCCTCCTCTTCCTT