| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.166093 |
| Chromosome: | chromosome_1 |
| Location: | 834656 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g004250 | TCTEX1,DLT3,TCT1 | Flagellar inner arm dynein light chain, Tctex1 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGGAACGTCTTCTTAAAGAGCTTCAGCG |
| Internal bar code: | CTGGTAAGGCAGTCCTGGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 99.7061 |
| LEAP-Seq n confirming: | 1696 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCGCTGACAATTCACT |
| Suggested primer 2: | GCACGATGGACTGTGCTAAA |