| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166103 |
| Chromosome: | chromosome 2 |
| Location: | 2764232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093975 | (1 of 1) PTHR23139:SF9 - SPLICING FACTOR U2AF 65 KDA SUBUNIT | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGCCGGCTGCAGGGCCCTCCGTCGCG |
| Internal bar code: | GCAGCAGGAAATGTGCGGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 96.4187 |
| LEAP-Seq n confirming: | 1400 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTAAGCAGTTAACCTCG |
| Suggested primer 2: | TCCACCTCCATTCCTACTGC |