| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166107 |
| Chromosome: | chromosome 16 |
| Location: | 5165069 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683400 | (1 of 10) PTHR10174 - RETINALDEHYDE BINDING PROTEIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGACGTCAACCCCAAGCTGGCCAAGG |
| Internal bar code: | ACGGAGGTGTGGACGGGACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 99.6071 |
| LEAP-Seq n confirming: | 6592 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGAACCCGCTTACATGG |
| Suggested primer 2: | TAAGGGTGCTCGACTTGCTT |