Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.166130 |
Chromosome: | chromosome_13 |
Location: | 3741337 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589450 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTAAGCAAAACACAACTCGCTTTCACTCC |
Internal bar code: | GGCTCGTCTGTTATCCTGAATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 716 |
LEAP-Seq percent confirming: | 99.7695 |
LEAP-Seq n confirming: | 3030 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACATGCCTGGTTGTAAGCC |
Suggested primer 2: | GAGGTAGCTGAACTCCACCG |