| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.166164 |
| Chromosome: | chromosome 11 |
| Location: | 1927668 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468350 | (1 of 1) K00486 - kynurenine 3-monooxygenase (KMO) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTCCCCGGAGGTTGGGTATGCGAGATA |
| Internal bar code: | CTATATTGTTTTTGGGGTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 99.5667 |
| LEAP-Seq n confirming: | 3677 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTCCGTTGCACACACAC |
| Suggested primer 2: | TGGTCGGGTAGTTAGGTTGC |