| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.166189 |
| Chromosome: | chromosome 6 |
| Location: | 5403659 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283900 | (1 of 1) PTHR16007//PTHR16007:SF15 - EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED // TRANSMEMBRANE PROTEIN 45B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGATTGGCTGCTAAACCCGGCCCGCGTG |
| Internal bar code: | TTGTAAGGAAGGCTCCCACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 93.7341 |
| LEAP-Seq n confirming: | 9559 |
| LEAP-Seq n nonconfirming: | 639 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTATCTTTTCCTTGTGC |
| Suggested primer 2: | GCAGGGCGTGTATAACCTGT |