| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166198 |
| Chromosome: | chromosome 9 |
| Location: | 3644291 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390356 | (1 of 1) PTHR22849//PTHR22849:SF0 - WDSAM1 PROTEIN // WD REPEAT, SAM AND U-BOX DOMAIN-CONTAINING PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACCATGCCGGTGCCGCACCTGCTCGTG |
| Internal bar code: | GCCGCATTGCCTAGGCCGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 99.7538 |
| LEAP-Seq n confirming: | 9318 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGGGTACGTCCTGCTAC |
| Suggested primer 2: | CGTCTAGATAGTCGAGGGCG |