Insertion junction: LMJ.RY0402.166200_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.166200
Chromosome:chromosome 1
Location:3835105
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g024800 (1 of 2) PF00059//PF07714//PF14295 - Lectin C-type domain (Lectin_C) // Protein tyrosine kinase (Pkinase_Tyr) // PAN domain (PAN_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTATACACCACGTGACATCGTCCTGTAA
Internal bar code:TAGGGCTAGGAACAAGAGTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:749
LEAP-Seq percent confirming:99.4835
LEAP-Seq n confirming:8860
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CACATTGTCGGGCTTAAGGT
Suggested primer 2:AATCATCGTAGGCTTCGTGG