| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166208 |
| Chromosome: | chromosome 8 |
| Location: | 936623 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361850 | (1 of 1) PF05981 - CreA protein (CreA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACTTGTACTAATAATCTTGGGCAAGCC |
| Internal bar code: | CCGTCCTCGGTCGTTGTGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 994 |
| LEAP-Seq percent confirming: | 97.4967 |
| LEAP-Seq n confirming: | 2181 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCCACCTACCGACAACA |
| Suggested primer 2: | ACACGCACATGCAATGTTTT |