| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166218 |
| Chromosome: | chromosome 13 |
| Location: | 3589656 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588310 | MRPL47,uL29m,MRPL29 | Mitochondrial ribosomal protein L29; (1 of 1) K17428 - large subunit ribosomal protein L47 (MRPL47, NCM1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCTTTCATCCTCGCCCTCGTCCACTCC |
| Internal bar code: | ACGTGATCGACCTGGCGTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 292 |
| LEAP-Seq percent confirming: | 96.4706 |
| LEAP-Seq n confirming: | 738 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAACGTCTCAGGTGGTGT |
| Suggested primer 2: | TGTGGCCATCAACAACAACT |