| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166218 |
| Chromosome: | chromosome 13 |
| Location: | 3589666 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588310 | MRPL47,uL29m,MRPL29 | Mitochondrial ribosomal protein L29; (1 of 1) K17428 - large subunit ribosomal protein L47 (MRPL47, NCM1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTCTGGAGCGCCTTCGTTTGCCAATTG |
| Internal bar code: | GTGGTCTATCCTACCTGCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1048 |
| LEAP-Seq percent confirming: | 99.7164 |
| LEAP-Seq n confirming: | 1758 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAACGTCTCAGGTGGTGT |
| Suggested primer 2: | TGTGGCCATCAACAACAACT |