| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166265 |
| Chromosome: | chromosome 2 |
| Location: | 7387179 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144802 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTATCCGGCTGTCTGGCGCTGGCTACCGC |
| Internal bar code: | ACCCTTTGACAGTCGAGATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1009 |
| LEAP-Seq percent confirming: | 68.709 |
| LEAP-Seq n confirming: | 314 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTATGCATGGACCGAC |
| Suggested primer 2: | CTTGCCTGCGATGTGTAGAA |