| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166267 |
| Chromosome: | chromosome 3 |
| Location: | 5848168 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189400 | TSS2,SERRS2 | Seryl-tRNA(Sec) synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCACACGGCAAACGGCGGTGGCTGGCT |
| Internal bar code: | AATATACAGGAGGATTGGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 43 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCAGGAGAAGAGCAAG |
| Suggested primer 2: | CTCCTTCCTGCACTCTGTCC |