| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166338 |
| Chromosome: | chromosome 6 |
| Location: | 7259401 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298326 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATTCATCAGACGTCCAGCTGCCTCTTC |
| Internal bar code: | CACAAACACGGAATGGAAGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 98.8355 |
| LEAP-Seq n confirming: | 2037 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAGCCTTTCACATCCAT |
| Suggested primer 2: | AACAGTCAGCTCCCCTAGCA |