| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166344 |
| Chromosome: | chromosome 4 |
| Location: | 3560464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228400 | WRK1 | (1 of 1) PF03106 - WRKY DNA -binding domain (WRKY); WRKY family transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCCACATGAGGCTTCCGCCAAGTCACC |
| Internal bar code: | GCGCCGTTCTCGGCGCGGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 99.903 |
| LEAP-Seq n confirming: | 4121 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACACGGGAGAACAGACAT |
| Suggested primer 2: | GCAACTTAGCCGACTTGACC |