| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166357 |
| Chromosome: | chromosome 12 |
| Location: | 4471365 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521600 | EEP7 | (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos); Endonuclease/exonuclease/phosphatase family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAGTGAAGGCGTAGCGGCGGGCGGGGG |
| Internal bar code: | ATGTAGGCCGCCGATCACCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1109 |
| LEAP-Seq percent confirming: | 97.4622 |
| LEAP-Seq n confirming: | 7220 |
| LEAP-Seq n nonconfirming: | 188 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGGTCCTAGTTTCTCGGT |
| Suggested primer 2: | CCCTGACAAACACCACACAC |