Insertion junction: LMJ.RY0402.166364_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.166364
Chromosome:chromosome 2
Location:3241460
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095076 ROC39,MFT10 Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGGGGGCTAGGCTCTAGTTGGGTAGGGA
Internal bar code:GTGGCTTTCGCCGGAGGCTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:671
LEAP-Seq percent confirming:94.9173
LEAP-Seq n confirming:2409
LEAP-Seq n nonconfirming:129
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGCTTGCACAGTATGAT
Suggested primer 2:CCATCAACAACACCAACAGC