| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.166365 |
| Chromosome: | chromosome 14 |
| Location: | 533178 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611484 | (1 of 4) IPR023485 - Phosphotyrosine protein phosphatase I superfamily | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGAGAGCTCTGCATTTCTCAAACCGGG |
| Internal bar code: | GACTGCCGCGACGCCGCGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 99.8516 |
| LEAP-Seq n confirming: | 2692 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGGGTTTATGGTTGACT |
| Suggested primer 2: | GCGGCTAGGTAGGATAAGGC |