| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166369 |
| Chromosome: | chromosome 1 |
| Location: | 2461088 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013801 | SXD1,TCY1 | (1 of 1) 5.5.1.24 - Tocopherol cyclase; Tocopherol cyclase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCTCTCCTCCTCTTCTGCATTGGGTTC |
| Internal bar code: | GCGTATGGTCATGCACACAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 99.5532 |
| LEAP-Seq n confirming: | 2451 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGCAGTGGTGGACTCAG |
| Suggested primer 2: | TATATGGGTGCAATGAGCGA |