Insertion junction: LMJ.RY0402.166394_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.166394
Chromosome:chromosome 2
Location:3399693
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095105 (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTTCCTGCAAGGCGGAATGTCCACAGAT
Internal bar code:TTGTCACTCGCTCAATTGCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:560
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:225
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTTGCGCTTTGTTTTCCT
Suggested primer 2:GCAGACTCCGTAGCTTCCAC