| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166418 |
| Chromosome: | chromosome 16 |
| Location: | 6749100 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673169 | (1 of 3) PF03942 - DTW domain (DTW) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACGGCTCTGCGCAAGCTGCGAAGCGAC |
| Internal bar code: | GGCCCACGCCATCAGCTTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 913 |
| LEAP-Seq percent confirming: | 94.9495 |
| LEAP-Seq n confirming: | 1222 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGGAGAGGATGGAAGGG |
| Suggested primer 2: | GAACAGCACATACAGGGGGT |