Insertion junction: LMJ.RY0402.166445_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.166445
Chromosome:chromosome 2
Location:6492396
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g116350 (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAACCCACTTCGCTACCCCCCCCCTCCCC
Internal bar code:ACAGTGTGCCGCGGACTAAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:795
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGTGGCAGTGGATAGGG
Suggested primer 2:CCTACCCATGCCTACATGCT