Insertion junction: LMJ.RY0402.166453_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.166453
Chromosome:chromosome 3
Location:217807
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g144344 (1 of 1) IPR003613//IPR011009//IPR013083//IPR013320 - U box domain // Protein kinase-like domain // Zinc finger, RING/FYVE/PHD-type // Concanavalin A-like lectin/glucanase domain intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAACGCCCACACGCTACTCCATTGCACG
Internal bar code:TCTGGCGCTCGTAACCGTCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:594
LEAP-Seq percent confirming:91.982
LEAP-Seq n confirming:1021
LEAP-Seq n nonconfirming:89
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCCTCCATCTCCTCCATC
Suggested primer 2:GCAGACCCGTCCTTACCATA