| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166472 |
| Chromosome: | chromosome 3 |
| Location: | 1788002 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154200 | (1 of 1) PTHR15970//PTHR15970:SF2 - FAMILY NOT NAMED // ELL-ASSOCIATED FACTOR EAF | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGAAGACCCGATAAGACTGGAAATTGT |
| Internal bar code: | GAGTTCAGAGGTTAAAGGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 986 |
| LEAP-Seq percent confirming: | 98.6248 |
| LEAP-Seq n confirming: | 1004 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTCGATGAAGACCTGGG |
| Suggested primer 2: | CCTTGTCCTTGAGCTTCCTG |