| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166475 |
| Chromosome: | chromosome 8 |
| Location: | 62204 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358522 | CEP5 | Cysteine endopeptidase; (1 of 1) PTHR12411//PTHR12411:SF372 - CYSTEINE PROTEASE FAMILY C1-RELATED // PROTEIN TAG-329 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGCCAGGCAGAGTAGCTGCAGCGCGTG |
| Internal bar code: | AAGTCGAATGGGAGGCGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 99.7838 |
| LEAP-Seq n confirming: | 1846 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCATGAGTCTCTCTGAGC |
| Suggested primer 2: | AGTCAATCTCCCCTCGTCCT |