| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166488 |
| Chromosome: | chromosome 9 |
| Location: | 3648072 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390467 | CGL66 | (1 of 3) PTHR12385:SF44 - PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEIN; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGTGGAGCGTGTCCGGAAGCGCGTAG |
| Internal bar code: | GTGGATGCCCAGGCAGAAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 99.1184 |
| LEAP-Seq n confirming: | 787 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTGGAGAAGGACGTGGT |
| Suggested primer 2: | TTCATCCTGTCGTTCTGCTG |