Insertion junction: LMJ.RY0402.166508_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.166508
Chromosome:chromosome 7
Location:2659040
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g330650 (1 of 10) PF02536 - mTERF (mTERF) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGCATGTGCCTTCTGCCGACCCCCGCC
Internal bar code:CGAGCGCTAGGTAGGGAATGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:935
LEAP-Seq percent confirming:99.6979
LEAP-Seq n confirming:990
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCGGAGTAATTGATGATT
Suggested primer 2:GCACACTTATCGAGGGTGGT