| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166559 |
| Chromosome: | chromosome 2 |
| Location: | 9163361 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143507 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAAGAAAGTGGCTGAGACCCGCCGGTT |
| Internal bar code: | CGACGCGATTGTTCCTGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 87.2222 |
| LEAP-Seq n confirming: | 157 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTGACCCCATACCCCCT |
| Suggested primer 2: | CGTAGTGATGACGTGATGGG |