| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.166559 |
| Chromosome: | chromosome 2 |
| Location: | 9163425 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143507 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCGCACTCGGGACTACGGCAGCAGTAG |
| Internal bar code: | AGGGGTATAATTTCAAATCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 56.7217 |
| LEAP-Seq n confirming: | 481 |
| LEAP-Seq n nonconfirming: | 367 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTGACCCCATACCCCCT |
| Suggested primer 2: | GCGTAGCAAGAAGGGACAAG |