Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.166560 |
Chromosome: | chromosome 17 |
Location: | 2946105 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g719950 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAGCCAAACAGACAACTAGCTTTATGC |
Internal bar code: | TCGATAGCCCGACGTTACGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 822 |
LEAP-Seq percent confirming: | 99.6063 |
LEAP-Seq n confirming: | 1012 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCAGTCCACAGACCAGTCC |
Suggested primer 2: | CTTCGGTGCAGATGAGGTTT |