| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166568 |
| Chromosome: | chromosome 1 |
| Location: | 2959845 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018075 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCGAGGGCGCGTCCAGCGGCTGCGGGGC |
| Internal bar code: | TAATGGGTGTATGCGGTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 99.7322 |
| LEAP-Seq n confirming: | 2979 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCTGGCGATAGAGTACA |
| Suggested primer 2: | ACTCGGGACTTTGCTTCTCA |