| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166579 |
| Chromosome: | chromosome_3 |
| Location: | 3757476 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g169900 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTAAGTACGCAATGCACAGTTATTTGGT |
| Internal bar code: | GCGTGACATGTATCGCAGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 919 |
| LEAP-Seq percent confirming: | 99.736 |
| LEAP-Seq n confirming: | 4911 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGGTGAGGCGACTGTGT |
| Suggested primer 2: | GACAGCCTGCTCCTATACGC |