| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166585 |
| Chromosome: | chromosome 10 |
| Location: | 5437664 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458450 | GPX5,GPXH | Glutathione peroxidase 5; (1 of 4) 1.11.1.9 - Glutathione peroxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGTGTCCCATCGTGGGATCCCAGCCAA |
| Internal bar code: | CGACGATGTTGCGCTAGCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 98.3392 |
| LEAP-Seq n confirming: | 1125 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAGCAGTCGAGCATTGT |
| Suggested primer 2: | AACCAATCGCCTAACACCTG |