Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.166596 |
Chromosome: | chromosome 14 |
Location: | 3351788 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g630100 | RPL13 | Ribosomal protein L13, component of cytosolic 80S ribosome and 6 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCTGCTAGACTGGTGGAGTTGCACTTA |
Internal bar code: | GGGTGCGTAGATCACGATTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 812 |
LEAP-Seq percent confirming: | 98.725 |
LEAP-Seq n confirming: | 542 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAACGCCAGTAATAGCACA |
Suggested primer 2: | CAAGCTTCAGCAACAATGGA |