Insertion junction: LMJ.RY0402.166596_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.166596
Chromosome:chromosome 14
Location:3351788
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g630100 RPL13 Ribosomal protein L13, component of cytosolic 80S ribosome and 6 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGCTGCTAGACTGGTGGAGTTGCACTTA
Internal bar code:GGGTGCGTAGATCACGATTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:812
LEAP-Seq percent confirming:98.725
LEAP-Seq n confirming:542
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACGCCAGTAATAGCACA
Suggested primer 2:CAAGCTTCAGCAACAATGGA